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The study presents an integrated geospatial and machine learning framework to assess forest and biodiversity vulnerability in Assam, India, under projected climate stress scenarios. Using multi-source remote sensing datasets and climate variables, the work applies GIS-based spatial modeling and machine learning algorithms to identify climate hotspots, evaluate forest resilience, and highlight biodiversity-rich yet highly vulnerable landscapes. The findings underscore how data-driven approaches can inform adaptive forest management, enhance biodiversity conservation, and support climate-resilient policy frameworks in India’s ecologically fragile regions.